Rule 1. Fatty acid, C12 to C24
Malassezia has no gene for fatty acid synthase, so it cannot build fatty acids and has to take
them from outside. That was found in the genome of M. globosa
(Xu and colleagues, 2007) and then in
all 14 species (Wu and colleagues, 2015).
In the study the range comes from, the yeast grew only when fatty acids from C12 to C24 were
added, and every acid tested in that range supported growth except elaidic and nervonic acid
(Porro and colleagues, 1976).
Matches lauric, myristic, palmitic, stearic, oleic, linoleic and the other named acids from C12 to
C24, and their salts. Evidence: genome studies and one in vitro growth study.
Rule 2. Ester of a C12 to C24 fatty acid
M. furfur split the fatty acid esters it was given and grew on the acids released. How well it
did depended on the alcohol half of the ester: ethyl esters were split best, then isopropyl, and
decyl oleate only a little
(Mayser and colleagues, 1995). A
later test of ten emulsifiers found every strain grew on PEG-7 glyceryl cocoate, PEG glyceryl
stearate and macrogol-50 stearate, while PEG-35 castor oil was used only by M. furfur
(Mayser and colleagues, 1997).
Matches names such as isopropyl myristate, glyceryl stearate, PEG-100 stearate, cetyl palmitate and
sorbitan oleate, plus jojoba, which is a liquid wax made of long-chain esters. Evidence: in vitro.
Rule 3. Polysorbate
Polysorbates 20, 40, 60 and 80 are sold as Tween, and labs use them as a lipid source to tell
the species apart (Guého and
colleagues, 1996). That is also why this rule is the weakest of the four. In one
identification study, M. furfur used all four, while M. globosa and M. restricta, two of the
species the authors call clinically important, used none of them as their only lipid
(Kaneko and colleagues, 2007).
Matches polysorbate 20, 40, 60, 80 and their close variants. Evidence: in vitro, varies by species.
Rule 4. Plant or animal oil or butter
Plant and animal oils and butters are mostly triglycerides. Malassezia makes lipases that split
triglycerides into free fatty acids
(Ro and Dawson, 2005), and whole
M. globosa cells split both diolein and triolein
(DeAngelis and colleagues, 2007).
Grown under olive oil, M. sympodialis and the other newer species released large amounts of free
fatty acids from it
(Mayser and colleagues, 1998).
Matches seed, kernel, nut and fruit oils, butters and triglycerides. It leaves out mineral oil,
silicones and essential oils, which are not triglycerides. Evidence: a review, in vitro work and
human scalp samples.